Researcher Database

（Last updated : 2024-02-22 11:00:07）　

ASANO Shinji

Department / Course College of Pharmaceutical Sciences Department of Pharmacy

Title / Position Professor

Other Affiliations
1.	Research Organization of Science and Technology Research Center for Drug Discovery and Development Scieces

Academic background
1.		│ Kyoto University Ph.D. (Pharmaceutical Sciences)
2.	～1982/03	Faculty of Pharmaceutical Science │ Kyoto University │ Graduated
3.	～1984/03	Master Course │ Biochemistry │ Graduate School, Division of Pharmaceutical Sciences │ Kyoto University │ Completed

Research activities
1.		The Pharmaceutical Society of Japan
2.		The Japanese Biochemical Society
3.		American Physiological Society
4.		American Society for Biochemistry and Molecular Biology
5.		The Membrane Society of Japan
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Subject of research
1.	Discovery of drugs involved in functional regulation of airway ciliary cells.
2.	Study on the function and expression of ACE2, a receptor for SARS-Cov2 on airway ciliary cells.
3.	Construction of primary culture cell system of ependymal cells.

Research summary
1. Discovery of drugs involved in functional regulation of airway ciliary cells. 2. Study on the function and expression of ACE2, a receptor for SARS-Cov2 on airway ciliary cells. 1. Discovery of drugs involved in functional regulation of airway ciliary cells We Inhale 20,000 liters of air in a day. Inhaled foreign materials and particulate matters are trapped by mucous layer covering the surface of airway, and excreted outside the body by ciliary movement. This system called “mucociliary clearance” is important biological defense mechanism of airway and lung, which prevents us from being infected by viruses, bacteria, and particulate matters such as PM2.5. In the previous study, we isolated mouse and human nasal, airway, and lung ciliary cells, and observed their ciliary movement by using high-speed cameras. At present, we search for drugs which improve mucociliary clearance. 2. Study on the function and expression of ACE2, a receptor for SARS-Cov2 on airway ciliary cells. New coronavirus, SARS-Cov2 infects human cells by binding its viral protein to angiotensin converting enzyme 2 (ACE2) located on the cell surface of human airway ciliary cells. We have our working hypothesis that SARS-Cov2 infects ciliary cells, loses their ciliary movement, and impairs mucociliary clearance, which results in more compromised situation and exacerbation of medical condition. We are now studying the effects of molecules targeting ACE2 on ciliary movement as well as intracellular environment ciliary cells. (3) Ependymal cells covering the surface of the ventricles contain motile cilia. Cerebrospinal fluid secreted from choroid plexus is circulated in the brain by the movement of ependymal cilia. In fact, ventriculomegaly (dilation of the cerebral ventricles) and hydrocephalus are observed when the movement of ependymal cilia is impaired. However, there are many unknown parts about the mechanism of ciliogenesis as well as the regulation mechanism of movement of ependymal cilia. We constructed the primary culture system of ependymal cilia from newborn mice and are studying the molecular mechanism of ciliogenesis. The regulation mechanism of cilia movement is also studied.

Present specialized field
Functional Biochemistry, Biological pharmacy, General Physiology (Keyword：Biochemistry, Molecular biology, Molecular physiology)

Books
1.	2002	Gastric Proton Pump, Alpha- and Beta- Subunit. "Mechanism and Consequences of Proton Transport │ ,pp.59-70
2.	2000	A chimeric H+,K+-ATPase Inhibitable with Both Ouabain and SCH 28080. "Na/K-ATPase and Related ATPases │ ,pp.163-168
3.	2000	Chimeric analysis of beta-subunits between H+,K+-ATPase and Na+,K+-ATPase for the functional expression of gastric H+,K+-ATPase. "Na/K-ATPase and Related ATPases │ ,pp.241-244
4.	1998	Bioactive diterpenoids from a tropical herb, Scoparia dulcis L. "Towards Natural Medicine Research in the 21st Century │ ,pp.197-202
5.	1997	Gastric H+, K+-ATPase; Its Functional Expression and Site-Directed Mutagenesis. │ ,pp.307-315
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Papers
1.	2024/02	Inhibition of ezrin phosphorylation by NSC305787 attenuates procaterol-stimulated ciliary beating in airway cilia. │ Biochemical and Biophysical Research Communications │ 703 (149685)
2.	2022	Ezrin knockdown reduces procaterol-stimulated ciliary beating without morphological changes in mouse airway cilia. │ Journal of Cell Science │ 135 (jcs259201)
3.	2022/05	Hochu-ekki-to enhanced airway ciliary beating by an [Ca2+]i increase via TRPV4 in mice. │ Phytomedicine Plus │ 2 (2),pp.100243
4.	2022	Secondary bile acid lithocholic acid attenuates neutrally evoked ion transport in the rat distal colon. │ Biomedical Research │ 43,pp.223-239
5.	2022	Minimum biological domain of xenin-25 required to induce anion secretion in the rat ileum. │ Peptides │ 147 (17680)
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Academic conference presentation
1.	2021/12	ACE2 in the primary culture of human nasal and bronchial epithelial cells.
2.	2021/12	Research on the regulatory factors involved in differentiation of multiciliated cells.
3.	2021/12	Roles of ezrin in regulation of ciliary beating in airway ciliary cells.
4.	2021/11	Transforming growth factor inhibits differentiation into ependymal multiciliated cells in vitro.
5.	2020/03	Activation of airway ciliary beating by an [Ca2+]i increased via nifedipine-sensitive Ca2+ channels stimulated by amboxol.
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