Researcher Database

（Last updated : 2022-07-04 15:41:16）

TAKADA Tatsuyuki

Department / Course College of Pharmaceutical Sciences Department of Pharmaceutical Sciences

Title / Position Professor

Other Affiliations
1.	Graduate School of Pharmacy
2.	Research Organization of Science and Technology Research Center for Drug Discovery and Development Scieces
3.	Research Organization of Science and Technology The Bio Medical Engineering Research Center

Academic background
1.	～1988/03	Graduate School, Division of Agriculture │ Tohoku University

Research activities
1.		International Society for Stem Cell Research
2.		The Molecular Biology Society of Japan

Subject of research
1.	Differentiation control of pluripotent stem cells Effect of environmental chemicals on cell differentiation signals Preservation of endemic species in Lake Biwa using stem cell biology Molecular cell biology in endemic species

Research summary
Differentiation control of pluripotent stem cells and its application in pharmaceutical sciences I am focusing on the differentiation mechanism of pluripotent stem cells such as ES, iPS cells and germ stem cells with primary emphasis on retinoic acid signal and effect of chemicals on the signal transduction to control their differentiation. My research also includes validation of natural compounds and synthetic chemicals on cell differentiation and carcinogenesis using genetically engineered stem cells. Recent data suggest that adipocyte differentiation can be switched to osteoblast simply using siRNA. This method can be applied for the treatment and prevention of osteoporosis. In addition, we found that environmental chemicals modulate germ cell differentiation and retinoic acid signaling in pluripotent stem cells. I am also interested in the application of stem cell biology on preservation of endemic species of lake Biwa which is one of the ancient lake in the world. We succeeded to establish cell line from endemic fish (Gnathopogon caerulescens) and to recapitulate entire spermatogenesis to differentiate fertile sperm from spermatogonia in vitro.

Present specialized field
Applied molecular and cellular biology, Developmental Biology, Conservation of biological resources, Risk sciences of radiation and chemicals, Biological System Pharmaceutical Science, Applied Genome Science (Keyword：Stem cells, pluripotency, differentiation, retinoic acid signal, environment, endemic species, fish)

Books
1.	2008	多機能細胞から脂肪細胞への運命決定メカニズム │ ,pp.13-18

Papers
1.	2018	Cryopreservation of male and female gonial cells by vitrification in the critically endangered cyprinid honmoroko Gnathopogon caerulescens │ Fish Physiol. Biochem │ 44(2),pp.503-513 (Co-authored)
2.	2017/10	Successful vitrification of whole juvenile testis in the critically endangered cyprinid honmoroko (Gnathopogon caerulescens) │ Zygote │ 25 (5),pp.652-661 (Co-authored)
3.	2017/02	In vitro differentiation of fertile sperm from cryopreserved spermatogonia of the endangered endemic cyprinid honmoroko (Gnathopogon caerulescens) │ Sci Rep. │ ,pp.7,42852 (Co-authored)
4.	2016/01	DNA methylation dynamics in mouse preimplantation embryos revealed by mass spectrometry │ Sci. Rep. │ 6:19134 (Co-authored)
5.	2016	Decreased DNA Methylation in the Shati/ Nat8l Promoter in Both Patients with Schizophrenia and a Methamphetamine- Induced Murine Model of Schizophrenia-Like Phenotype │ PLoS ONE │ ,pp.e0157959 (Co-authored)
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Academic conference presentation
1.	2019/07/19	Seasonal analysis of gonadal transcriptome in endemic cyprinid Honmoroko (Gnathopogon caerulescens) (52nd Annual Meeting of the Society for the Study of Reproduction)
2.	2017/09/29	In vitro production of fertile sperm from cryopreserved spermatogonia of the endangered endemic cyprinid honmoroko (Gnathopogon caerulescens) (Fourth World Congress of Reproductive Biology)
3.	2017/07/13	In vitro differentiation of fertile sperm from cryopreserved spermatogonia of the endangered endemic cyprinid honmoroko (Gnathopogon caerulescens) (50th Annual Meeting of the Society for the Study of Reproduction)
4.	2016/02/17	DNA methylation dynamics in mouse preimplantation embryos revealed by mass spectrometry International Symposium on Epigenome Dynamics and Regulation in Germ Cells (International Symposium on Epigenome Dynamics and Regulation in Germ Cells)
5.	2015/09/19	Flow Cytometric Analysis of Testicular Cells in Endangered Endemic Cyprinid Honmoroko (Gnathopogon caerulescens) (21st Japanese Medaka and Zebrafish Meeting)
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Grants-in-Aid for Scientific Research -KAKENHI-
1.	2002 ～ 2005	Establishment of embryonic gem cells and their differentiation into germ cells.