マツムラ ヒロヨシ
松村 浩由
MATSUMURA HIROYOSHI
所属 生命科学部 生物工学科
職名 教授
言語種別 英語
発行・発表の年月 2020/09
形態種別 研究論文(学術雑誌)
標題 Crystal structure of a GH1 β-glucosidase from Hamamotoa singularis.
執筆形態 共著
掲載誌名 Protein science : a publication of the Protein Society
掲載区分 国外
巻・号・頁 29(9),2000-2008
著者・共著者 Uehara Ryo, Iwamoto Riki, Aoki Sayaka, Yoshizawa Takuya, Takano Kazufumi, Matsumura Hiroyoshi, Tanaka Shun-Ichi
概要 A GH1 β-glucosidase from the fungus Hamamotoa singularis (HsBglA) has high transgalactosylation activity and efficiently converts lactose to galactooligosaccharides. Consequently, HsBglA is among the most widely used enzymes for industrial galactooligosaccharide production. Here, we present the first crystal structures of HsBglA with and without 4'-galactosyllactose, a tri-galactooligosaccharide, at 3.0 and 2.1 Å resolutions, respectively. These structures reveal details of the structural elements that define the catalytic activity and substrate binding of HsBglA, and provide a possible interpretation for its high catalytic potency for transgalactosylation reaction.
DOI 10.1002/pro.3916
ISSNコード 1469-896X
PMID 32713015