Department / Course College of Life Sciences Department of Biotechnology
Title / Position Professor
Language English
Publication Date 2020/09
Type Research paper (scientific journal)
Title Crystal structure of a GH1 β-glucosidase from Hamamotoa singularis.
Contribution Type Joint Work
Journal Protein science : a publication of the Protein Society
Journal Type Another Country
Volume, Issue, Page 29(9),2000-2008
Author and coauthor Uehara Ryo, Iwamoto Riki, Aoki Sayaka, Yoshizawa Takuya, Takano Kazufumi, Matsumura Hiroyoshi, Tanaka Shun-Ichi
Details A GH1 β-glucosidase from the fungus Hamamotoa singularis (HsBglA) has high transgalactosylation activity and efficiently converts lactose to galactooligosaccharides. Consequently, HsBglA is among the most widely used enzymes for industrial galactooligosaccharide production. Here, we present the first crystal structures of HsBglA with and without 4'-galactosyllactose, a tri-galactooligosaccharide, at 3.0 and 2.1 Å resolutions, respectively. These structures reveal details of the structural elements that define the catalytic activity and substrate binding of HsBglA, and provide a possible interpretation for its high catalytic potency for transgalactosylation reaction.
DOI 10.1002/pro.3916
ISSN 1469-896X
PMID 32713015