マツムラ ヒロヨシ
松村 浩由
MATSUMURA HIROYOSHI
所属 生命科学部 生物工学科
職名 教授
言語種別 英語
発行・発表の年月 2020/11
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 Hybrid Rubisco with Complete Replacement of Rice Rubisco Small Subunits by Sorghum Counterparts Confers C4 Plant-like High Catalytic Activity.
執筆形態 共著
掲載誌名 Molecular plant
掲載区分 国外
巻・号・頁 13(11),1570-1581
著者・共著者 Matsumura Hiroyoshi, Shiomi Keita, Yamamoto Akito, Taketani Yuri, Kobayashi Noriyuki, Yoshizawa Takuya, Tanaka Shun-Ichi, Yoshikawa Hiroki, Endo Masaki, Fukayama Hiroshi
概要 In this study, we knocked out rice RbcS multigene family using the CRISPR-Cas9 technology and completely replaced rice RbcS with sorghum RbcS in rice Rubisco. Obtained hybrid Rubisco showed almost C4 plant-like catalytic properties, i.e., higher kcat, higher Kc, and lower Sc/o. Transgenic lines expressing the hybrid Rubisco accumulated reduced levels of Rubisco, whereas they showed slightly but significantly higher photosynthetic capacity and similar biomass production under high CO2 condition compared with wild-type rice. High-resolution crystal structural analysis of the wild-type Rubisco and hybrid Rubisco revealed the structural differences around the central pore of Rubisco and the βC-βD hairpin in RbcS. We propose that such differences, particularly in the βC-βD hairpin, may impact the flexibility of Rubisco catalytic site and change its catalytic properties.
DOI 10.1016/j.molp.2020.08.012
ISSNコード 1752-9867
PMID 32882392