タケダ ヨウイチ
武田 陽一
TAKEDA YOICHI
所属 生命科学部 生物工学科
職名 教授
言語種別 日本語
発行・発表の年月 2020/12
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 Chemical-Synthesis-Based Approach to Glycoprotein Functions in the Endoplasmic Reticulum.
執筆形態 共著
掲載誌名 Chemistry (Weinheim an der Bergstrasse, Germany)
掲載区分 国外
巻・号・頁 26(67),15461-15470
著者・共著者 Ito Yukishige, Kajihara Yasuhiro, Takeda Yoichi
概要 The introduction of Asn-linked glycans to nascent polypeptides occurs in the lumen of the endoplasmic reticulum of eukaryotic cells. After the removal of specific sugar residues, glycoproteins acquire signals in the glycoprotein quality control (GPQC) system and enter the folding cycle composed of lectin-chaperones calnexin (CNX) and calreticulin (CRT), glucosidase II (G-II), and UDP-Glc:glycoprotein glucosyltransferase (UGGT). G-II initiates glycoproteins' entry and exit from the cycle, and UGGT serves as the "folding sensor". This account summarizes our effort to analyze the properties of enzymes and lectins that play important roles in GPQC, especially those involved in the CNX/CRT cycle. To commence our study, general methods for the synthesis of high-mannose-type glycans and glycoproteins were established. Based on these, various substrates to analyze components of the GPQC were created, and properties of CRT, G-II, and UGGT have been clarified.
DOI 10.1002/chem.202004158
ISSNコード 1521-3765
PMID 33107166