マンネン タロウ
萬年 太郎
MANNEN TARO
所属 生命科学部 生命医科学科
職名 助教
言語種別 英語
発行・発表の年月 2021/09
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 Distinct RNA polymerase transcripts direct the assembly of phase-separated DBC1 nuclear bodies in different cell lines.
執筆形態 共著
掲載誌名 Molecular biology of the cell
掲載区分 国外
巻・号・頁 mbcE21020081
担当区分 筆頭著者 , 責任著者
著者・共著者 Mannen Taro, Goto Masato, Yoshizawa Takuya, Yamashita Akio, Hirose Tetsuro, Hayano Toshiya
概要 The mammalian cell nucleus is a highly organized organelle that contains membrane-less structures referred to as nuclear bodies (NBs). Some NBs carry specific RNA types that play architectural roles in their formation. Here, we show two types of RNase-sensitive DBC1-containing NBs: DBC1 nuclear body (DNB) in HCT116 cells and Sam68 nuclear body (SNB) in HeLa cells that exhibit phase-separated features and are constructed using RNA polymerase I or II transcripts in a cell type-specific manner. We identified additional protein components present in DNB by immunoprecipitation-mass spectrometry, some of which (DBC1 and HNRNPL) are required for DNB formation. The rescue experiment using the truncated HNRNPL mutants revealed that two RNA-binding domains and intrinsically disordered regions of HNRNPL play significant roles in DNB formation. All these domains of HNRNPL promote in vitro droplet formation, suggesting the need for multivalent interactions between HNRNPL and RNA as well as proteins in DNB formation.
DOI 10.1091/mbc.E21-02-0081
ISSNコード 1939-4586
PMID 34495685