モトムラ カズキ
元村 一基
MOTOMURA KAZUKI
所属 総合科学技術研究機構
職名 助教
言語種別 日本語
発行・発表の年月 2012/05
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 The role of decapping proteins in the miRNA accumulation in Arabidopsis thaliana.
執筆形態 共著
掲載誌名 RNA biology
掲載区分 国外
巻・号・頁 9(5),644-52
著者・共著者 Motomura Kazuki, Le Quy Tn, Kumakura Naoyoshi, Fukaya Takashi, Takeda Atsushi, Watanabe Yuichiro
概要 Decapping 1 (DCP1), Decapping 2 (DCP2) and VARICOSE (VCS) are components of the decapping complex that removes the 7-methyl-guanosine 5'-diphosphate from the 5' end of mRNAs. In animals, the decapping proteins are involved in miRNA-mediated gene silencing, whereas in plants the roles of the decapping proteins in the miRNA pathway are not well understood. Here we demonstrated that the accumulation of miRNAs decreased in dcp1, dcp2 and vcs mutants, indicating that DCP1, DCP2 and VCS are important for the miRNA pathway in Arabidopsis thaliana. The primary miRNAs (pri-miRNAs) did not increase and miRNA biogenesis components did not decrease in these mutants, suggesting that the miRNA decrease in decapping mutants is not due to the defect of pri-miRNA processing. We showed that the accumulation of miRNA targets increased concomitantly with the decrease of miRNA in the decapping mutants. Our results suggested that the seedling lethal phenotypes in the dcp1, dcp2 and vcs mutants are caused not only by the defect in decapping, but also by the disruption of miRNA-mediated gene regulation.
DOI 10.4161/rna.19877
ISSNコード 1555-8584
PMID 22614834