モトムラ カズキ
元村 一基
MOTOMURA KAZUKI
所属 総合科学技術研究機構
職名 助教
言語種別 日本語
発行・発表の年月 2018/01
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 A pharmacological study of Arabidopsis cell fusion between the persistent synergid and endosperm.
執筆形態 共著
掲載誌名 Journal of cell science
掲載区分 国外
巻・号・頁 131(2)
著者・共著者 Motomura Kazuki, Kawashima Tomokazu, Berger Frédéric, Kinoshita Tetsu, Higashiyama Tetsuya, Maruyama Daisuke
概要 Cell fusion is a pivotal process in fertilization and multinucleate cell formation. A plant cell is ubiquitously surrounded by a hard cell wall, and very few cell fusions have been observed except for gamete fusions. We recently reported that the fertilized central cell (the endosperm) absorbs the persistent synergid, a highly differentiated cell necessary for pollen tube attraction. The synergid-endosperm fusion (SE fusion) appears to eliminate the persistent synergid from fertilized ovule in Arabidopsis thaliana Here, we analyzed the effects of various inhibitors on SE fusion in an in vitro culture system. Different from other cell fusions, neither disruption of actin polymerization nor protein secretion impaired SE fusion. However, transcriptional and translational inhibitors decreased the SE fusion success rate and also inhibited endosperm division. Failures of SE fusion and endosperm nuclear proliferation were also induced by roscovitine, an inhibitor of cyclin-dependent kinases (CDK). These data indicate unique aspects of SE fusion such as independence of filamentous actin support and the importance of CDK-mediated mitotic control.
DOI 10.1242/jcs.204123
ISSNコード 1477-9137
PMID 28808086