モトムラ カズキ
元村 一基
MOTOMURA KAZUKI
所属 総合科学技術研究機構
職名 助教
言語種別 日本語
発行・発表の年月 2021/04
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex.
執筆形態 共著
掲載誌名 Nature communications
掲載区分 国外
巻・号・頁 12(1),2331
担当区分 筆頭著者
著者・共著者 Motomura Kazuki, Takeuchi Hidenori, Notaguchi Michitaka, Tsuchi Haruna, Takeda Atsushi, Kinoshita Tetsu, Higashiyama Tetsuya, Maruyama Daisuke
概要 During the double fertilization process, pollen tubes deliver two sperm cells to an ovule containing the female gametes. In the pollen tube, the vegetative nucleus and sperm cells move together to the apical region where the vegetative nucleus is thought to play a crucial role in controlling the direction and growth of the pollen tube. Here, we report the generation of pollen tubes in Arabidopsis thaliana whose vegetative nucleus and sperm cells are isolated and sealed by callose plugs in the basal region due to apical transport defects induced by mutations in the WPP domain-interacting tail-anchored proteins (WITs) and sperm cell-specific expression of a dominant mutant of the CALLOSE SYNTHASE 3 protein. Through pollen-tube guidance assays, we show that the physiologically anuclear mutant pollen tubes maintain the ability to grow and enter ovules. Our findings provide insight into the sperm cell delivery mechanism and illustrate the independence of the tip-localized vegetative nucleus from directional growth control of the pollen tube.
DOI 10.1038/s41467-021-22661-8
ISSNコード 2041-1723
PMID 33888710