ブン ショウホウ
文 小鵬
WEN XIAOPENG
所属 薬学部 創薬科学科
職名 助教
言語種別 英語
発行・発表の年月 2021/05
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 Evaluation of the Effects of Solvents Used in the Fabrication of Microfluidic Devices on Cell Cultures.
執筆形態 共著
掲載誌名 Micromachines
掲載区分 国外
巻・号・頁 12(5)
著者・共著者 Wen Xiaopeng, Takahashi Seiichiro, Hatakeyama Kenji, Kamei Ken-Ichiro
概要 Microfluidic microphysiological systems (MPSs) or "organs-on-a-chip" are a promising alternative to animal models for drug screening and toxicology tests. However, most microfluidic devices employ polydimethylsiloxane (PDMS) as the structural material; and this has several drawbacks. Cyclo-olefin polymers (COPs) are more advantageous than PDMS and other thermoplastic materials because of their low drug absorption and autofluorescence. However, most COP-based microfluidic devices are fabricated by solvent bonding of the constituent parts. Notably, the remnant solvent can affect the cultured cells. This study employed a photobonding process with vacuum ultraviolet (VUV) light to fabricate microfluidic devices without using any solvent and compared their performance with that of solvent-bonded systems (using cyclohexane, dichloromethane, or toluene as the solvent) to investigate the effects of residual solvent on cell cultures. Quantitative immunofluorescence assays indicated that the coating efficiencies of extracellular matrix proteins (e.g., Matrigel and collagen I) were lower in solvent-bonded COP devices than those in VUV-bonded devices. Furthermore, the cytotoxicity of the systems was evaluated using SH-SY5Y neuroblastoma cells, and increased apoptosis was observed in the solvent-processed devices. These results provide insights into the effects of solvents used during the fabrication of microfluidic devices and can help prevent undesirable reactions and establish good manufacturing practices.
DOI 10.3390/mi12050550
ISSNコード 2072-666X
PMID 34066183