アブドアルカーディル ルディ
ABDALKADER RODI
ABDALKADER RODI
所属 立命館グローバル・イノベーション研究機構
職名 助教
言語種別 日本語
発行・発表の年月 2017/11
形態種別 研究論文(学術雑誌)
査読 査読あり
標題 The development of mechanically formed stable nanobubbles intended for sonoporation-mediated gene transfection.
執筆形態 共著
掲載誌名 Drug delivery
掲載区分 国外
巻・号・頁 24(1),320-327
担当区分 筆頭著者
著者・共著者 Abdalkader Rodi, Kawakami Shigeru, Unga Johan, Higuchi Yuriko, Suzuki Ryo, Maruyama Kazuo, Yamashita Fumiyoshi, Hashida Mitsuru
概要 In this study, stable nano-sized bubbles (nanobubbles [NBs]) were produced using the mechanical agitation method in the presence of perfluorocarbon gases. NBs made with perfluoropropane had a smaller size (around 400 nm) compared to that of those made with perfluorobutane or nitrogen gas. The lipid concentration in NBs affected both their initial size and post-formulation stability. NBs formed with a final lipid concentration of 0.5 mg/ml tended to be more stable, having a uniform size distribution for 24 h at room temperature and 50 h at 4 °C. In vitro gene expression revealed that NBs/pDNA in combination with ultrasound (US) irradiation had significantly higher transfection efficacy in colon C26 cells. Moreover, for in vivo gene transfection in mice left limb muscles, there was notable local transfection activity by NBs/pDNA when combined with US irradiation. In addition, the aged NBs kept at room temperature or 4 °C were still functional at enhancing gene transfection in mice. We succeeded in preparing stable NBs for efficient in vivo gene transfection, using the mechanical agitation method.
DOI 10.1080/10717544.2016.1250139
ISSNコード 1521-0464
PMID 28165819